Degumming of vegetable oils by a novel phospholipase B from Pseudomonas fluorescens BIT-18 |
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Authors: | Jiang Fangyan Wang Jinmei Kaleem Imdad Dai Dazhang Zhou Xiaohong Li Chun |
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Affiliation: | a School of Life Science, Beijing Institute of Technology, 100081 Beijing, PR China b School of Chemistry and Chemical Engineering, Shihezi University, 832003 Shihezi Xinjiang, PR China |
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Abstract: | Pseudomonas fluorescens BIT-18 was isolated from soil near a vegetable oil factory and shown to produce a B-type phospholipase. The enzyme was partially purified by ammonium sulfate precipitation. Gas chromatography demonstrated that the enzyme preparation hydrolyzed both the 1- and 2-ester bonds of phosphatidylcholine. When degumming of soybean, rapeseed, and peanut oil was performed with this enzyme preparation, oils with phosphorous contents lower than 5 mg/kg were obtained after 5 h of enzyme treatment at 40 °C. The enzyme preparation did not show lipase activity, thus free fatty acids were only generated from the phospholipids. Therefore, this novel phospholipase B is potentially useful for the refining of high-quality oils with attractive yields. |
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Keywords: | PLA1, phospholipase A1 PLA2, phospholipase A2 PLB, phospholipase B PLC, phospholipase C LAT, lipid acyl transferase 1-palmitoyl-2-oleoyl PC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine |
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