Fluorographic detection of [3H]thymidine-labeled deoxyribonucleic acid using agarose gels infused with 2,5-diphenyloxazole prior to electrophoresis |
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| Authors: | J W Ludlow K C Yuen R A Consigli |
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| Institution: | 1. Laboratory for Machine Tools and Production Engineering (WZL) of RWTH Aachen University, Campus-Boulevard 30, 52074 Aachen, Germany;2. Fraunhofer Institute for Production Technology IPT, Steinbachstr. 17, 52074 Aachen, Germany |
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| Abstract: | A fluorographic procedure for the detection of 3H]thymidine-labeled deoxyribonucleic acids electrophoresed in agarose gels was developed. 2,5-Diphenyloxazole (PPO) was added to the agarose solution before pouring of the gel for electrophoresis. This procedure did not interfere with the electrophoretic mobility of the DNA molecules. The radioactive detection efficiency was found to be improved over an existing procedure whereby the agarose gel was infused with PPO after electrophoresis with the aid of acetic acid. |
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