外生菌根真菌对‘NL-895杨’光合作用的影响

以‘NL-895杨’(Populus×euramericanacv.Nanlin 895)幼苗为材料,研究6种外生菌根真菌接种对其幼苗光合作用的影响.结果显示:接种60 d后‘NL-895杨’叶片的叶绿素a和b含量、PSⅡ最大光能转化效率(Fv/Fm)比对照极显著提高,其中叶绿素a和b的增加幅度分别为28.16%～68.03%和69.29%～138.26%,并以接种劣味乳菇的‘NL-895杨’Fv/Fm最高,且与对照差异极显著.各处理菌根苗培养150 d时‘NL-895杨’的净光合速率表现为美味牛肝菌>红绒盖牛肝菌>劣味乳菇>彩色豆马勃2号>紫金蜡蘑>彩色豆马勃1号>对照;菌根真菌处理对‘NL-895杨’叶片胞间CO2浓度影响较小,但其蒸腾速率显著高于对照,而且能促进总糖的合成与积累,并以接种彩色豆马勃2号的‘NL-895杨’苗总糖含量最高.研究表明,接种菌根真菌可以有效提高‘NL-895杨’对光能资源的利用率,增加其光合产物积累,显著促进其生长.

Effect of Ectomycorrhizal Fungi on Photosynthesis of Poplar NL-895

Populus×euramericana cv.Nanlin 895 seedlings were inoculated with six kinds of ectomycorrhizal fungi to study the effect on photosynthesis.The results showed that poplar leaf chlorophyll a,b and the maximal photochemical efficiency(Fv/Fm) of PSⅡ of ‘NL-895’ were significantly higher than that of the control.The increase of chlorophyll a and b were 28.16% to 68.03%,and 69.29% to 138.26%,respectively.The NL-895 seedlings vaccinated by Lactarius insulsus possessed maximum actual photochemical efficiency,significant differences with contrast;The net photosynthetic rate sequence of inoculated ‘NL-895’ seedlings was Boletus edulis>Xerocomus chrysenteron>Lactarius insulsus>Pisolithus tinctorius 2(Pt2)>Laccaria aemthystea>Pisolithus tinctorius 1(Pt1)>CK after 150 d cultivation.Mycorrhizal inoculation had little effect on intercellular CO2 concentration,but transpiration rate was significantly higher than that of control,and promoted total sugar synthesis and accumulation.Seedlings vaccinated by Pt2 possessed the highest sugar content.Mycorrhizal inoculation could promote utilization of solar heat resource,increase photosynthate,and accelerate growth significantly.