接种乳黄粘盖牛肝菌和荧光假单胞菌对油松苗生长及猝倒病的影响

在盆栽条件下研究了单接种外生菌根真菌乳黄粘盖牛肝菌(SL)、单接种荧光假单胞菌(PF)、双接种(SL+PF)和不接种菌剂(CK)4个处理对油松苗生长、盆栽土壤指标、油松苗生理生化指标及猝倒病发生的影响.结果显示:(1)不同接种处理的油松苗成活率、生长状况及根系活力均高于对照,且SL+PF促生效果最好,其成活率、苗高、地径、地上干重、总干重及根系活力分别比对照提高22.10%、36.16%、44.86%、124.14%、161.54%和100%.(2)双接种可同时有效提高土壤酶活性和土壤养分含量,土壤脲酶、碱性磷酸酶和蔗糖酶活性均与速效氮、速效磷和有机碳呈显著或极显著正相关.(3)接种病原菌前后油松苗的MDA含量表现为CK>PF>SL>SL+PF,SOD、CAT和PAL活性均为SL+PF>SL>PF>CK,但接种病原菌前后不同处理酶活性增长幅度不同,CAT和PAL活性均以SL+PF增幅最大,分别比对照增长76.18%和112.64%.(4)接种处理的油松苗猝倒病发病率及病情指数均低于对照,且以SL+PF抗病效果最好,分别比对照降低60.94%和70.55%.研究表明,接种菌根真菌可有效提高土壤酶活性和土壤养分含量,增强植株体内的抗氧化酶活性,从而促进油松苗生长,抑制猝倒病的发生,菌根促生菌荧光假单胞菌增强了菌根真菌的作用.

Effect of Inoculating Suillus lactifluus and Pseudomonas fluorescensto Pinus tabulaeformis Growth and Resistant of Damping-off

The effects of Suillus lactifluus and Pseudomonas fluorescens inoculation on Pinus tabulaeformis growth,soil pot index,plant physiological character and damping-off resistance under pot conditions were investigated.The treatments were inoculated S.lactifluus(SL),inoculated P.fluorescens(PF),simultaneously inoculated(SL+PF) and uninoculation(CK),respectively.The results showed that:(1)inoculated seedlings were higher than uninoculated control in the survival rate,growth rate and root activity,and the both in...