Effect of biological pretreatment with Trametes hirsuta yj9 on enzymatic hydrolysis of corn stover |
| |
Authors: | Feng-hui Sun Jiang Li Yue-xiang Yuan Zhi-ying Yan Xiao-feng Liu |
| |
Affiliation: | Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China |
| |
Abstract: | In this study, a newly isolated Trametes hirsuta yj9 was used to pretreat corn stover in order to enhance enzymatic digestibility. T. hirsuta yj9 preferentially degraded lignin to be as high as 71.49% after 42-day pretreatment. Laccase and xylanase was the major ligninolytic and hydrolytic enzyme, respectively and filter paper activity (FPA) increased gradually with prolonged pretreatment time. Sugar yields increased significantly after pretreatment with T. hirsuta yj9, reaching an enzymatic digestibility of 73.99% after 42 days of pretreatment. Scanning electron microscopy (SEM) showed significant structural changes in pretreated corn stover, the surface of pretreated corn stover became increasingly coarse, the gaps between cellulose fibers were visible, and many pores were developed. Correlation analysis showed that sugar yields were inversely proportional to the lignin contents, less related to cellulose and hemicellulose contents. |
| |
Keywords: | Biological pretreatment Corn stover Enzymatic hydrolysis Lignin Trametes hirsuta yj9 |
本文献已被 ScienceDirect 等数据库收录! |
|