Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor |
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Authors: | Cui Xi-Hua Murthy Hosakatte Niranjana Jin You-Xun Yim Yong-Hyeon Kim Ji-Yeong Paek Kee-Yoeup |
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Institution: | aResearch Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Chungju 361-763, Republic of Korea;bDepartment of Botany, Karnatak University, Dharwad 580 003, India;cCenter for Analytical Chemistry, Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science, Daejeon 305-340, Republic of Korea;dAmorepacific Corporation R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Republic of Korea |
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Abstract: | The effects of inoculum density, aeration volume and culture period on accumulation of biomass and secondary metabolites in adventitious roots of Hypericum perforatum in balloon type airlift bioreactors (3 l capacity) were investigated. The greatest increment of biomass as well as metabolite content occurred at an inoculum density of 3 g l−1 and an aeration volume of 0.1 vvm. After 6 weeks of culture, an approximately 50-fold increase in biomass was recorded containing 60.11 mg g−1 dry weight (DW) of phenolics, 42.7 mg g−1 DW of flavonoids and 0.80 mg g−1 DW of chlorogenic acid. Liquid chromatography–mass spectroscopy/mass spectroscopy demonstrated that the presence of quercetin and hyperoside in adventitious roots at a level of 1.33 and 14.01 μg g−1 DW, respectively after 6 weeks of culture. The results suggest scale-up of adventitious root culture of H. perforatum for the production of chlorogenic acid, quercetin and hyperoside is feasible. |
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Keywords: | Adventitious roots Balloon type bioreactor Bioactive compounds Hypericum perforatum |
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