Reactivity of bacterial and fungal laccases with lignin under alkaline conditions |
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Authors: | Moya Raquel Saastamoinen Päivi Hernández Manuel Suurnäkki Anna Arias Enriqueta Mattinen Maija-Liisa |
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Institution: | aDepartamento de Microbiología y Parasitología, Universidad de Alcalá, Ctra. Madrid-Barcelona, Km 33.600, 28871 Alcalá de Henares, Madrid, Spain;bVTT Bioprocessing, P.O. Box 1000 (Tietotie 2), FI-02044 VTT, Finland |
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Abstract: | The ability of Streptomyces ipomoea laccase to polymerize secoisolariciresinol lignan and technical lignins was assessed. The reactivity of S. ipomoea laccase was also compared to that of low redox fungal laccase from Melanocarpus albomyces using low molecular mass p-coumaric, ferulic and sinapic acid as well as natural (acetosyringone) and synthetic 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) mediators as substrates. Oxygen consumption measurement, MALDI-TOF MS and SEC were used to follow the enzymatic reactions at pH 7, 8, 9 and 10 at 30 °C and 50 °C. Polymerization of lignins and lignan by S. ipomoea laccase under alkaline reaction conditions was observed, and was enhanced in the presence of acetosyringone almost to the level obtained with M. albomyces laccase without mediator. Reactivities of the enzymes towards acetosyringone and TEMPO were similar, suggesting exploitation of the compounds and low redox laccase in lignin valorization under alkaline conditions. The results have scientific impact on basic research of laccases. |
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Keywords: | Abbreviations: ACN acetonitrile Aromatic &ndash OH aromatic hydroxyls ESI-MS/MS tandem electrospray ionization mass spectrometry HMR 7-hydroxymatairesinol HW hardwood LGF lignofibre LMS laccase-mediator system MaL Melanocarpus albomyces laccase MALDI-TOF MS matrix assisted laser desorption ionization time-of-flight mass spectrometry MM molecular mass MS-Lac laccase from Mycelia sterilia YY-5 MR matairesinol O S organosolv SEC size exclusion chromatography SECO secoisolariciresinol SilA Streptomyces ipomoea laccase TaL Thielavia arenaria laccase TEMPO 2 2 6 6-tramethylpiperidine 1-oxyl ThL Trametes hirsuta laccase TFA trifluoroacetic acid SW softwood |
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