Production of lipases by solid state fermentation using vegetable oil-refining wastes |
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Authors: | Santis-Navarro Angélica Gea Teresa Barrena Raquel Sánchez Antoni |
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Institution: | Composting Research Group, Department of Chemical Engineering, Escola d’Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain |
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Abstract: | Lipases were produced by a microbial consortium derived from a mixture of wastewater sludges in a medium containing solid industrial wastes rich in fats, under thermophilic conditions (temperature higher than 45 °C for 20 days) in 4.5-L reactors. The lipases were extracted from the solid medium using 100 mM Tris–HCl, pH 8.0 and a cationic surfactant agent (cetyltrimethylammonium chloride). Different doses of surfactant and buffer were tested according to a full factorial experimental design. The extracted lipases were most active at 61–65 °C and at pH 7.7–9. For the solid samples, the lipolytic activity reached up to 120,000 UA/g of dry matter. These values are considerably higher than those previously reported in literature for solid-state fermentation and highlight the possibility to work with the solid wastes as effective biocatalysts. |
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Keywords: | Solid-state fermentation Lipase Thermostability Organic wastes Sewage sludge |
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