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Induction of cytolytic activity of lymphocytes from carcinomatous pleural effusion by IL-2 and autologous tumor cells |
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| Authors: | Motonori Sairenji Shunsuke Yanoma Hisahiko Motohashi Osamu Kobayashi Kenzou Okada Takashi Okamoto Mamoru Tsukuda Makoto Umeda |
| Institution: | (1) Department of Surgery, Kanagawa Cancer Center, Nakaocho, Asahiku, 241 Yokohama;(2) Clinical Research Institute, Kanagawa Cancer Center, Nakaocho, Asahi-ku, 241 Yokohama;(3) Kihara Institute for Biological Research, Yokohama City University, Nakamura-cho, Minami-ku, 232 Yokohama |
| Abstract: | We established a cell line (STKM-1) from tumor cells obtained from carcinomatous pleural effusion of a gastric cancer patient. The lymphocytes separated from her peripheral blood or pleural effusion were cryopreserved and immunological experiments were performed after the establishment of the cell line. They were treated with IL-2 or with both IL-2 and mitomycin C (MMC)-treated autologous STKM-1 cells. The cytolytic activity against STKM-1 cells was elevated in lymphocytes cultured with IL-2, and was more prominently augmented in lymphocytes cultured with both IL-2 and MMC-treated STKM-1 cells. The elevation in cytolytic activity was more marked with pleural effusion lymphocytes than with the peripheral blood lymphocytes. The results suggest that the lymphocytes obtained from the pleural effusion would be an excellent source for adoptive immunotherapy.Abbreviations IL-2 interleukin-2 - LAK lymphokine activated killer - MLTC mixed lymphocyte tumor cell culture - MMC mitomycin C - MoAbs monoclonal antibodies - TIL tumor infiltrating lymphocytes |
| Keywords: | Gastric carcinoma Pleural effusion Mixed-lymphocyte-tumor-culture Autologous tumor Adoptive immunotherapy |
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