Isolation and characterization of microsatellite loci in <Emphasis Type="Italic">Melicope quadrilocularis</Emphasis> (Rutaceae), an endemic plant species of the Bonin Islands,Japan, and cross-species amplification in closely related taxa |
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| Authors: | Saeko Katoh Shingo Kaneko Yuji Isagi Noriaki Murakami Hidetoshi Kato |
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| Institution: | (1) Department of Biological Sciences, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan;(2) Graduate School of International Development and Cooperation, Hiroshima University, Kagamiyama 1-5-1, Higashi-Hiroshima 739-8529, Japan;(3) Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan |
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| Abstract: | Twelve microsatellite loci were isolated and characterized for Melicope quadrilocularis, an insular endemic tree species of the Bonin Islands. The observed number of alleles at each locus ranged from 1 to 18.
The range of expected heterozygosity was 0.0000–0.9445. The inter-specific applicability of these loci was evaluated by analyzing
two other endemic species and one endemic variety of Melicope that are also distributed on the Bonin Islands. All primer pairs for the 12 loci tested successfully amplified the loci in
all taxa, except for primers for four loci in M. nishimurae. |
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| Keywords: | Cross-amplification Melicope Microsatellite loci Bonin (Ogasawara) Islands |
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