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Long-term nutrient and water utilization during micropropagation of Cattleya on a liquid/membrane system
Authors:Adelberg  Jeffrey W  Desamero  Nenita V  Andrew Hale  S  Young  Roy E
Institution:(1) Department of Horticulture, Clemson University, Clemson, South Carolina USA, 29634;(2) PhilRice Institute, Nuera, Ecija, Philippines;(3) Biological and Agricultural Engineering Department, North Carolina State University, Raleigh, NC USA, 27695;(4) Agricultural and Biological Engineering, Department, Clemson University, Clemson, South Carolina USA, 29634
Abstract:Growth of two Cattleya clones (Sophrolaeliocattleya Jewel Box 'Scheherezade' and Brassolaeliocattleya Rugley's Mill 'Mendenhall') over 6 or 8 months on microporous polypropylene membrane rafts was compared for several inorganic nutrient formulations: Murashige and Skoog at full, half and quarter strength; Hoagland's hydroponic medium; and three media formulated specifically for orchid culture (Knudson, Lindemann, and Vacin and Went). Greatest fresh weight and number of plants per vessel were obtained on Murashige and Skoog medium and dilutions thereof. Shoot growth was greater than root growth only on full strength MS media. Media uptake by tissue and evaporation from the vessels required additional water to maintain contact between membrane and liquid. Most rapid growth occurred in periods following addition of water. In more dilute media formulations, shifting growth from shoots to roots was a possible outcome of low concentrations of ammonium.
Keywords:ammonium  mass flow  nutrient deficiency  orchids
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