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Protoplast fusion enhances lignocellulolytic enzyme activities in Trichoderma reesei |
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| Authors: | Yu-xiao Cui Jia-jing Liu Yan Liu Qi-yue Cheng Qun Yu Xin Chen Xiao-dong Ren |
| Affiliation: | 1. School of Life Science, Jilin University, Changchun, 130023, China 2. Beijing Institute of Transfusion Medicine, Beijing, 100850, China 4. Changchun University of Chinese Medicine, Changchun, 130117, China 3. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, 130023, China |
| Abstract: | Protoplast fusion was used to obtain a higher production of lignocellulolytic enzymes with protoplast fusion in Trichoderma reesei. The fusant strain T. reesei JL6 was obtained from protoplast fusion from T. reesei strains QM9414, MCG77, and Rut C-30. Filter paper activity of T. reesei JL6 increased by 18 % compared with that of Rut C-30. β-Glucosidase, hemicellulase and pectinase activities of T. reesei JL6 were also higher. The former activity was 0.39 Uml?1, while those of QM9414, MCG77, and Rut C-30 were 0.13, 0.11, and 0.16 Uml?1, respectively. Pectinase and hemicellulase activities of JL6 were 5.4 and 15.6 Uml?1, respectively, which were slightly higher than those of the parents. The effects of corn stover and wheat bran carbon sources on the cellulase production and growth curve of T. reesei JL6 were also investigated. |
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