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Secretion of biologically active human interleukin 22 (IL-22) by Lactococcus lactis |
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| Authors: | María J Loera-Arias Julio Villatoro-Hernández Miguel A Parga-Castillo Alejandro Salcido-Montenegro Oralia Barboza-Quintana Gerardo E Muñoz-Maldonado Roberto Montes-de-Oca-Luna Odila Saucedo-Cárdenas |
| Institution: | 1. Departamento de Histología, Facultad de Medicina, Universidad Autónoma de Nuevo León (UANL), Madero y Aguirre Peque?o s/n Mitras Centro, 66460, Monterrey, Nuevo León, Mexico 2. Health and Nutrition Division, Chr-Hansen, B?ge Alle 10, 2970, H?rsholm, Denmark 3. Servicio de Anatomía Patológica y Citopatología, Hospital Universitario Dr José Eleuterio González de la Facultad de Medicina, UANL, Monterrey, N.L., Mexico 4. Servicio de Cirugía General del Hospital Universitario Dr José Eleuterio González de la Facultad de Medicina, UANL, Monterrey, N.L., Mexico 5. División de Genética, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social (IMSS), Monterrey, N.L., Mexico |
| Abstract: | Interleukin-22 (IL-22) participates in the modulation of innate immunity and inflammation. This cytokine has important therapeutic potential, such as with ulcerative colitis, liver and lung injury, and infection, in different animal models. We generated a Lactococcus lactis strain that secretes human IL-22 under the regulation of the nisin-inducible promoter. Identification and secretion of this cytokine was demonstrated using western blots of culture supernatants from IL-22-expressing bacteria. The recombinant IL-22 protein produced by L. lactis was biologically active as determined by its ability to induce IL-10 secretion when co-cultured with a colon epithelial cell line in vitro. We consider this novel strain a promising live vaccine for various therapeutic applications. |
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