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Identification and germline transformation of the ribosomal protein rp21 gene of Drosophila: complementation analysis with the Minute QIII locus reveals nonidentity
Authors:Mark A Kay  J -Y Zhang and Marcelo Jacobs-Lorena
Institution:(1) Department of Developmental Genetics and Anatomy, School of Medicine, Case Western Reserve University, 2119 Abington Rd, 44106 Cleveland, OH, USA;(2) Present address: Department of Pediatrics, Baylor College of Medicine, Texas Medical Center, 77030 Houston, TX, USA;(3) Present address: Department of Biochemistry, Tianjing Medical College, Tianjing, People's Republic of China
Abstract:Summary Minute loci represent a class of about 50 different Drosophila genes that appear to be functionally related. These genes may code for components of the protein synthetic apparatus. While one Minute locus has been recently shown to code for a ribosomal protein, it is not yet known whether any of the other Minute loci also code for ribosomal proteins. We have addressed this question by a combined molecular and genetic approach. In this report, a cloned DNA encoding the ribosomal protein rp21 is partially characterized. The rp21 gene maps to the same region (region 80 of chromosome 3L) as the temperature-sensitive Minute QIII gene. Using P-element mediated transformation, the rp21 gene was transformed into the germline of Drosophila. RNA blot experiments revealed that the transformed gene is expressed in transgenic flies. However, genetic complementation analysis indicated that the QIII locus and the rp21 gene are not identical. Implications of these findings for the relationship between Minutes and ribosomal protein genes are discussed.
Keywords:Drosophila  Minute  Ribosomal protein  Transformation
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