Angiogenic potential of CD44+ CD90+ multipotent CNS stem cells in vitro |
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Authors: | Oishi Kazuhiko Ito-Dufros Yuko |
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Institution: | Department of Pharmacology, Meiji Pharmaceutical University, Tokyo 204-8588, Japan. oishikz@my-pharm.ac.jp |
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Abstract: | Although it is now clear that several subpopulations of neural stem cells (NSCs) exist during early development and adulthood, the angiogenic potential of NSCs remains a subject of debate. Here, we report that CD44(+) CD90(+) cells isolated from primary neurospheres can form vascular-tube structures in vitro. NSCs isolated from the mouse embryonic cortex formed neurospheres when cultured in serum-free medium containing 20ng/ml basic fibroblast growth factor (bFGF). CD44(+) CD90(+) cells were enriched from the neurospheres using an EPICS ALTRA flow cytometer, and antibodies against CD44 and CD90. The purified CD44(+) CD90(+) cells generated neurospheres, and differentiated into neurons and astrocytes. When the cells were inoculated into collagen gels and cultured with 20% fetal bovine serum plus bFGF for 7 days, vascular tube-like structures were formed. These results indicate that CD44(+) CD90(+) cells have the ability to generate neurospheres and to form vascular tubes. |
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Keywords: | CD44 CD90 Neural stem cells Vascular-tube formation |
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