大麦DNA导入小麦产生抗白粉病变异的遗传研究

本研究将抗白粉病的大麦DNA通过花粉管途径直接导入感病的小麦品种花76中，后代出现13株抗白粉病变异株。其中5株在以后的世代中抗性稳定，另8株则继续分离。第2带分离株系的抗病株形成的第3代株系（或株行）中，抗性有分离的株行与无分离的株行比例为1.9:1，而分离株行内抗病株与不抗病株之比为3.35:1。抗性稳定株系与感病亲本杂交，F1表现高抗病，再与感病亲本回交，后代抗感病株比例为1:1，自交F2的比例为2.8:1。说明所获得的抗白粉病性受一对完全显性基因控制，抗病为显性。与已知抗白粉病基因的比较表明，这个抗病基因可能是来自大麦的一个新基因。13 Variant plants with immunity and high-resistance to powdery mildew were found in D1 generation from introducing resistant barley DNA into susceptible wheat cultivar, through pollen tube pathway after self pollination.Of the variants, 5 plants for the resistance had been stable and the other 8 plants segregated insuccessive generation.The ratio of segregating and stable plant-rows was 1.9:1 in D3 plant-rows derived from resistant plants of segregating D2-lines,and the ratio of resistant plants and susceptible plants was 3.35:1 among the segregating D3 plant-rows.The F1 -plants from crosses between stable resistant variants and susceptible parents were higgh resistant to powdery mildew.The ratio of resistant and susceptible plants was 1:1 in progenies of backcross of the F1 and susceptible parents, and this ratio was 2.8:1 in the F2 generation from the F1 selfing. Thus it can be seen that the resistance obtained is camtrolled by a pair of genes, the resistance is dominant. The results in comparison with known powdery mildew resistance genes in wheat indicated that the resistant gene obtained would be a new one from barley.

Inheritance of Powdery Mildew Resistance in Variants Obtained by Introducing Barley DNA into Wheat

13 Variant plants with immunity and high-resistance to powdery mildew were found in D1 generation from introducing resistant barley DNA into susceptible wheat cultivar, through pollen tube pathway after self pollination. Of the variants, 5 plants for the resistance had been stable and the other 8plants segregated in suecessive generation. The ratio of segregating and stab1e plant-rows was 1.9:1 inD3 plant-rows derived from resistant plants of segregating D2-lines, and the ratio of resistant plants andsusceptible plants was 3.35 :1 among the segregating D3 plant-rows. The F1-plants from crosses between stable resistant variants and susceptible parents were high resistant to powdery mildew. The ratio ofresistant and susceptible plants was 1:1 in progenies of backcross of the F1 and susceptib1e parents, andthis ratlo was 2.8:1 in the F2 gencration from the F1 selfing. Thus it can be scen that the resistance ob-tained is comtrolled by a pair of genes, the resistance is dominant. The results in comparison with knownpowdery mildew rcsistance genes in wheat indicated that the resistance gene obtained would be a new onefrom barley.