Environmental regulation of stomatal response in the <Emphasis Type="Italic">Arabidopsis</Emphasis> Cvi-0 ecotype |
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Authors: | Keina Monda Juntaro Negi Atsuhiro Iio Kensuke Kusumi Mikiko Kojima Mimi Hashimoto Hitoshi Sakakibara Koh Iba |
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Institution: | (1) Department of Biology, Faculty of Sciences, Kyushu University, Fukuoka 812-8581, Japan;(2) National Institute for Environmental Studies (NIES), Tsukuba 305-8506, Japan;(3) RIKEN Plant Science Center, Yokohama 230-0045, Japan; |
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Abstract: | The Arabidopsis Cape Verde Islands (Cvi-0) ecotype is known to differ from other ecotypes with respect to environmental stress responses.
We analyzed the stomatal behavior of Cvi-0 plants, in response to environmental signals. We investigated the responses of
stomatal conductance and aperture to high CO2] in the Cvi-0 and Col-0 ecotypes. Cvi-0 showed constitutively higher stomatal conductance and more stomatal opening than
Col-0. Cvi-0 stomata opened in response to light, but the response was slow. Under low humidity, stomatal opening was increased
in Cvi-0 compared to Col-0. We then assessed whether low humidity affects endogenous ABA levels in Cvi-0. In response to low
humidity, Cvi-0 had much higher ABA levels than Col-0. However, epidermal peels experiments showed that Cvi-0 stomata were
insensitive to ABA. Measurements of organic and inorganic ions in Cvi-0 guard cell protoplasts indicated an over-accumulation
of osmoregulatory anions (malate and Cl−). This irregular anion homeostasis in the guard cells may explain the constitutive stomatal opening phenotypes of the Cvi-0
ecotype, which lacks high CO2]-induced and low humidity-induced stomatal closure. |
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