Peroxisomal localisation of the final steps of the mevalonic acid pathway in<Emphasis Type="Italic"> planta</Emphasis> |
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Authors: | Andrew J Simkin Grégory Guirimand Nicolas Papon Vincent Courdavault Insaf Thabet Olivia Ginis Sadok Bouzid Nathalie Giglioli-Guivarc’h Marc Clastre |
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Institution: | 1.EA 2106, Biomolécules et Biotechnologies Végétales,Université Fran?ois-Rabelais de Tours,Tours,France;2.Laboratoire de Biotechnologie et Physiologie Végétale, Département des Sciences Biologiques,Faculté des Sciences de Tunis,Tunis,Tunisia |
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Abstract: | In plants, the mevalonic acid (MVA) pathway provides precursors for the formation of triterpenes, sesquiterpenes, phytosterols
and primary metabolites important for cell integrity. Here, we have cloned the cDNA encoding enzymes catalysing the final
three steps of the MVA pathway from Madagascar periwinkle (Catharanthus roseus), mevalonate kinase (MVK), 5-phosphomevalonate kinase (PMK) and mevalonate 5-diphosphate decarboxylase (MVD). These cDNA were
shown to functionally complement MVA pathway deletion mutants in the yeast Saccharomyces cerevisiae. Transient transformations of C. roseus cells with yellow fluorescent protein (YFP)-fused constructs reveal that PMK and MVD are localised to the peroxisomes, while
MVK was cytosolic. These compartmentalisation results were confirmed using the Arabidopsis thaliana MVK, PMK and MVD sequences fused to YFP. Based on these observations and the arguments raised here we conclude that the final
steps of the plant MVA pathway are localised to the peroxisome. |
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