Cytochemical localization of hydrogen peroxide generating sites in the rat thyroid gland |
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Authors: | Mary Anna Labato Richard T briggs |
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Institution: | 1. Department of Small Animal Medicine, Tufts University School of Veterinary Medicine. Angell Memorial Animal Hospital, Jamaica Plain, MA 02130 USA |
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Abstract: | Sites of H2O2 generation in lightly prefixed, intact thyroid follicles were studied by two cytochemical reactions: peroxidase-dependent DAB oxidation and cerium precipitation. In both cases reaction product accumulated on the apical surface of the follicle cell at the membrane-colloid interface. The former reaction was inhibited by the peroxidase inhibitor, aminotriazole; both reactions were blocked by the presence of catalase. NADH in the medium slightly increased the amount of cerium precipitation. The ferricyanide technique for oxidoreductase activity was also applied; reaction product again was associated with the apical surface. These results strongly imply that the follicle cells have a NADH oxidizing system generating H2O2 at the apical plasma membrane. |
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Keywords: | Hydrogen peroxide thyroid gland cytochemistry |
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