| 表达序列标签数据库搜索鉴定小鼠UBAP1基因及其数字化表达分析 |
| |
| 引用本文： | 钱骏,董利,张必成,王洁如,周鸣,李忠花,李伟芳,李小玲,李桂源. 表达序列标签数据库搜索鉴定小鼠UBAP1基因及其数字化表达分析[J]. 生物化学与生物物理进展, 2002, 29(2): 323-327 |
| |
| 作者姓名： | 钱骏 董利 张必成 王洁如 周鸣 李忠花 李伟芳 李小玲 李桂源 |
| |
| 作者单位： | 中南大学湘雅医学院肿瘤研究所,教育部癌变与侵袭原理重点实验室,长沙,410078 |
| |
| 基金项目： | 国家高技术“863”计划资助项目(102-10-01-05，Z19-01-01-03)、国家重点基础研究发展规划项目(973)(G1998051008)及美国中华医学基金资助(96655). |
| |
| 摘 要： | UBAP1(ubiquitin associated protein 1)基因是最近克隆的一个定位于人类染色体9p21-22鼻咽癌杂合性丢失高频区的泛肽相关蛋白家族新成员.为了深入研究UBAP1基因的功能，利用计算机对表达序列标签(expressed sequence tag, EST)、UniGene等数据库进行综合搜索分析，结合cDNA克隆测序的方法, 成功地获得了UBAP1基因在小鼠中的同源基因.小鼠UBAP1基因cDNA全长为2 676 bp，编码一个由441个氨基酸组成的蛋白质，在其蛋白质C端只有一个泛肽相关功能域（UBA domain）.与人UBAP1基因相比，两者编码的氨基酸序列有89%相同.基于EST的数字化表达分析显示UBAP1基因在小鼠正常组织中广泛高表达.
|
| 关 键 词： | UBAP1基因，基因克隆，表达序列标签，组织表达 |
| 收稿时间： | 2001-06-27 |
| 修稿时间： | 2001-06-27 |
Identification and Digitalized Expression Analysis of Murine UBAP1 Gene by Means of EST Database Searching |
| |
| QIAN Jun,DONG Li,ZHANG Bi-Cheng,WANG Jie-Ru,ZHOU Ming,LI Zhong-Hu,LI Wei-Fang,LI Xiao-Ling and LI Gui-Yuan. Identification and Digitalized Expression Analysis of Murine UBAP1 Gene by Means of EST Database Searching[J]. Progress In Biochemistry and Biophysics, 2002, 29(2): 323-327 |
| |
| Authors: | QIAN Jun DONG Li ZHANG Bi-Cheng WANG Jie-Ru ZHOU Ming LI Zhong-Hu LI Wei-Fang LI Xiao-Ling LI Gui-Yuan |
| |
| Affiliation: | Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China |
| |
| Abstract: | Ubiquitin associated protein 1(UBAP1) is a novel member of UBA domain protein family, located at human chromosome 9p21-22 where loss of heterozygosity frequently occurs in nasopharyngeal carcinoma. Through integrated analysis of public database such as EST, Unigene in silico and direct sequencing of cDNA clone, full-length cDNA sequence of murine homologoue of human UBAP1 gene was identified, which is 2 676 bp long and encodes a putative protein of 441 amino acids with a UBA domain. There is an average of 89% identical residues between mouse and human UBAP1 protein. Digitalized expression analysis based on EST data showed that mouse UBAP1 gene expressed ubiquitiously and strongly in most of mouse normal tissues. |
| |
| Keywords: | UBAP1 gene cloning expressed sequence tag(EST) tissue expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
|
点击此处可从《生物化学与生物物理进展》下载全文 |
|
