Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine |
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Authors: | Michal Margalith Adrián Vilalta |
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Institution: | 1. Vical Incorporated, 10390 Pacific Center Ct, San Diego, CA, 92121, USA
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Abstract: | Published data indicate that formulation of pDNA with cationic lipids could greatly enhance the response to a pDNA vaccine in larger mammals. The present work tested the influence of several pDNA:cationic lipid formulations on rabies neutralizing titers. Plasmid expressing Rabies G protein (CVS strain) was evaluated in vivo for ability to elicit neutralizing titers. pDNA:DMRIE-DOPE formulated at two DNA:cationic lipid molar ratios was compared in mice to a Vaxfectin?-pDNA formulation. Mouse data indicate that Vaxfectin? is more effective than DMRIE-DOPE in eliciting neutralizing titers. In addition, the ratio of pDNA to DMRIE-DOPE can also affect neutralizing titers. Our data show that sustained neutralizing titers (120 days) can be obtained after a single administration of DMRIE-DOPE-formulated pDNA in rabbits. |
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