Halotolerance studies onChlamydomonas reinhardtii: glycerol excretion by free and immobilized cells |
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Authors: | R. León F. Galván |
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Affiliation: | (1) Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Química, Universidad de Sevilla, Apartado 553, 41080- Sevilla, Spain |
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Abstract: | The freshwater green algaChlamydomonas reinhardtii can tolerate a maximum saline concentration of 200 mM NaCl. In response to this osmotic shock, the cells accumulated during the first 24 h 15% of the total glycerol synthesized as osmoregulatory metabolite, to provide the corresponding osmotic balance. After this period all glycerol synthesized was excreted to the medium, 4 g L-1 at 120 h in optimal conditions, before cell degradation occurred. This excretion was about 2-fold higher in Ca-alginate entrapped cells in the presence of 250 mM NaCl. It was concluded that immobilized cells may be of biotechnological interest for continuous glycerol photoproduction in air-lift bioreactors. |
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Keywords: | Alginate Chlamydomonas glycerol halotolerance immobilization |
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