Spectrophotometric measurement of plastoquinone photoreduction in the blue-green alga,Phormidium uncinatum |
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Authors: | Donat-P Häder Kenneth L Poff |
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Institution: | (1) Fachbereich Biologie-Botanik, Philipps-Universität, Lahnberge, D-3550 Marburg, Germany;(2) Plant Research Laboratory, Michigan State University, 48824 East Lansing, MI, USA |
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Abstract: | The redox state of plastoquinone was measured in vivo in the blue-green alga, Phormidium uncinatum by means of a double beam UV-spectrophotometer. The difference in absorbance of the oxidized and the reduced forms of plastoquinone was amplified, and stored and averaged in a computer. The redox state was changed by two alternating actinic light beams. When one actinic wavelength was kept constant at 700 nm (PSI) variation of the other yielded an action spectrum representing photosystem II. The inhibitors of the photosynthetic electron transport chain, DCMU and DBMIB, reduced the difference in absorbance between the oxidized and reduced forms of plastoquinone.Abbreviations DBMIB
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone
- DCMU
3-(3 ,4 -dichlorophenyl)-1, 1-dimethylurea |
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Keywords: | Phormidium uncinatum Photomovement Photosynthetic electron transport Plastoquinone UV-spectrophotometry |
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