| Ⅰ型单纯疱疹病毒糖蛋白G基因片段的克隆表达及初步鉴定 |
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| 引用本文： | 王洁,李越希,张云.Ⅰ型单纯疱疹病毒糖蛋白G基因片段的克隆表达及初步鉴定[J].中国生物工程杂志,2003,23(6):55-58. |
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| 作者姓名： | 王洁 李越希 张云 |
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| 作者单位： | 1. 南京医科大学公共卫生学院流行病与卫生统计学系, 南京210029;
2. 南京军区军事医学研究所, 南京210002 |
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| 摘 要： | 通过计算机分析Ⅰ型单纯疱疹病毒糖蛋白G的氨基酸序列,筛选出HSV1gG蛋白中优势抗原决定簇位点,用PCR技术扩增克隆含强抗原决定簇较集中的基因片段。将该段基因克隆至质粒表达载体pGEX4T2内,转化大肠杆菌TG1,构建成功了高效表达Ⅰ型单纯疱疹病毒糖蛋白G基因的工程菌。用纯化的表达蛋白HSV1gGGST作抗原ELISA分析证实有较好的抗原性和特异性,显示可应用于单纯疱疹病毒感染的诊断 。
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| 关 键 词： | 鉴定 单纯疱疹病毒 HSV1-gG 基因克隆 基因表达 |
Cloning,Expression of HSV-1 gG Gene Fragment and Identification of the Expressed Protein |
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| Wang Jie,Li Yuexi,Zhang Yun.Cloning,Expression of HSV-1 gG Gene Fragment and Identification of the Expressed Protein[J].China Biotechnology,2003,23(6):55-58. |
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| Authors: | Wang Jie Li Yuexi Zhang Yun |
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| Institution: | Wang Jie 1 Li Yuexi 2 Zhang Yun 2 (1 Department of Epidemiology & Biostatistics School of Public Health Nanjing Medical University Nanjing 210029) (2 Huadong Research Institute for Medical Biotechnics Nanjing 210002) |
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| Abstract: | The herpes simplex virus type 1 glycoprotein G gene fragment containing dominant antigenic determinants confirmed by analysing amino acid sequences was cloned by PCR and inserted into plasmid vector pGEX 4T 2,then the recombinant plasmid was transformed to E.coli TG1.The engineering E.coli expressing HSV1 gG/GST was established.The purified expressed protein showed good antigenicity and specificity by ELISA test.The study is of great significance in early diagnosis of HSV.\ |
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| Keywords: | Herpes simples virus Glycoprotein G Gene cloning Gene Expression Identification |
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