Cloning of <Emphasis Type="Italic">Bacillus licheniformis</Emphasis> Xylanase Gene and Characterization of Recombinant Enzyme |
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Authors: | Charles C Lee Rena E Kibblewhite-Accinelli Michael R Smith Kurt Wagschal William J Orts Dominic W S Wong |
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Institution: | USDA-ARS-WRRC, 800 Buchanan St., Albany, CA, 94710, USA, Charles.C.Lee@ars.usda.gov. |
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Abstract: | Hemicellulose is a major component of lignocellulose biomass. Complete degradation of this substrate requires several different enzymatic activities, including xylanase. We isolated a strain of Bacillus licheniformis from a hot springs environment that exhibited xylanase activity. A gene encoding a 23-kDa xylanase enzyme, Xyn11, was cloned, and the recombinant protein was expressed in an Escherichia coli host and biochemically characterized. The optimum activity of the enzyme was at pH 5-7 and 40-50 degrees C. The enzyme was stable at temperatures up to 50 degrees C. Against birchwood xylan, the enzyme had an apparent K ( m ) of 6.7 mg/mL and V (max) of 379 mumol/min/mg. |
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