Preparation method and growth factor content of platelet concentrate influence the osteogenic differentiation of bone marrow stromal cells |
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| Authors: | Francesca Perut Giuseppe Filardo Erminia Mariani Annarita Cenacchi Loredana Pratelli Valentina Devescovi Elizaveta Kon Maurilio Marcacci Andrea Facchini Nicola Baldini Donatella Granchi |
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| Institution: | 1. Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Rizzoli Orthopedic Institute, Bologna, Italy;2. Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopedic Institute, Bologna, Italy;3. Laboratory of Immunorheumatology and Tissue Regeneration, Rizzoli Orthopedic Institute, Bologna, Italy;6. Clinical Pathology Unit, Rizzoli Orthopedic Institute, Bologna, Italy;5. Immunohematology and Transfusion Medicine and Cell and Musculoskeletal Tissue Bank, Bologna, Italy;4. Department of Clinical Medicine, University of Bologna, Bologna, Italy;7. Department of Biomedical and Neuromotion Sciences, University of Bologna, Bologna, Italy;1. Department of Hematology, University Medical Center Groningen, Groningen, The Netherlands;2. Department of Internal Medicine, University Medical Center Groningen, Groningen, The Netherlands;3. Department of Laboratory Medicine, University Medical Center Groningen, Groningen, The Netherlands;4. Department of Cell Biology, University Medical Center Groningen, Groningen, The Netherlands;5. Department of Laboratory Medicine, Children''s and Women''s Health, Norwegian University of Science and Technology, Trondheim, Norway;1. Department of Pediatric Surgery, First People’s Hospital affiliated to Huzhou University Medical College, Huzhou, Zhejiang Province, China;2. Department of Pediatrics, First People’s Hospital affiliated to Huzhou University Medical College, Huzhou, Zhejiang Province, China;1. Department of Blood Transfusion, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China;2. Department of Blood Transfusion, Beijing Friendship Hospital, Capital Medical University, Beijing, China;3. Department of Blood Transfusion, The First Medical Center, Chinese PLA General Hospital, Beijing, China;1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Department of Oral and Maxillofacial Surgery, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran |
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| Abstract: | Background aimsAn extensive debate about the clinical benefits of autologous platelet concentrates used as a treatment option for patients with orthopedic injuries is ongoing. The aim of this study was to determine whether different compositions of platelet concentrates may affect the osteogenic differentiation of bone marrow stromal cells (BMSC).MethodsPure platelet-rich plasma (P-PRP) and leukocyte-PRP (L-PRP) were characterized for platelet and leukocyte content. As an indicative marker of the delivery of growth factors (GFs), the release of basic fibroblast growth factor (bFGF) from platelet gel (PG) was measured at 1, 18, 48 and 72 h and at 7 d. The ability of different PGs to induce proliferation and differentiation of BMSC was evaluated by using bioactivity assays.ResultsThe platelet recovery was significantly higher in L-PRP, either fresh or frozen. PGs derived from L-PRP and P-PRP showed significant differences in terms of bFGF release and biological activity. bFGF release was faster both in fresh and frozen L-PRP preparations. Moreover, L-PRP samples were able to induce a significantly higher proliferation of BMSC compared with P-PRP or PPP samples. Even though all PG preparations allowed the deposition of mineral nodules in BMSC cultures, the mineralization activity correlated significantly with bFGF levels.ConclusionsThe biological activity of platelet concentrates differs according to preparation technique, which affects platelet and leukocyte content and GF availability. Because GF levels are not always optimal in subjects with defective bone healing, composition and bioactivity of PRP should be analyzed to test the reliability and potential effectiveness of the regenerative treatment. |
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