Perilesional intrathecal administration of autologous bone marrow stromal cells achieves functional improvement in pigs with chronic paraplegia |
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| Authors: | Mercedes Zurita Concepcion Aguayo Celia Bonilla Alicia Rodriguez Jesus Vaquero |
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| Institution: | 1. Department of Radiation Oncology, UT Southwestern Medical Center, Dallas, USA;2. Department of Radiation Oncology, Radboud University Medical Center, Nijmegen, The Netherlands;3. Department of Biostatistics, University of California, Los Angeles, USA;4. Department of Radiology, University of California, Los Angeles, USA;5. Department of Radiology, UT Southwestern Medical Center, Dallas, TX, USA;1. Department of Radiology, Shanghai First People''s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People''s Republic of China;2. Department of Vascular Surgery, Shanghai Ninth People''s Hospital Affiliated to Shanghai Jiaotong University, School of Medicine, Shanghai, People''s Republic of China;3. Department of Plastic Surgery, Shanghai First People''s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People''s Republic of China;1. Department of Hepatobiliary Surgery, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, People''s Republic of China;2. Department of Radiology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, People''s Republic of China;3. Department of Pathology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, People''s Republic of China;1. Post-Graduation in Biotechnology, USP/IPT/I. Butantan, Avenida Prof. Lineu Prestes 2415, Cidade Universitária, 05508-900, São Paulo, SP, Brazil;2. UNESP – Univ Estadual Paulista, FCL, Department of Biological Science, Avenida Dom Antonio 2100, 19806-900, Assis, SP, Brazil;3. Department of Physiology, ECB, UNIFESP, Rua Botucatu 862, 04023-062, São Paulo, SP, Brazil;1. Hematology and Stem Cell Transplantation Section, Division of Hematology/Oncology, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA;2. Vanderbilt Asthma, Sinus, and Allergy Program (VASAP), Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA;3. Pediatric Stem Cell Transplant Program, Monroe Carell Jr. Children''s Hospital Vanderbilt University Medical Center, Nashville, Tennessee, USA |
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| Abstract: | Background aimsAt present, on the basis of the great number of preclinical studies and preliminary clinical trials in humans, bone marrow stromal cell (BMSC) transplantation offers promise in the treatment of paraplegia. Nevertheless, there is not enough experience in humans about the best candidates for this type of cell therapy or details about the best parameters or best route of administration.MethodsTwo adult paraplegic pigs with chronic paraplegia were treated only with perilesional intrathecal administration of 40 × 106 autologous BMSC suspended in autologous plasma and followed for 1 year after cell transplantation.ResultsOur study showed clinical improvement, starting at 2 mo after BMSC administration and reaching stabilization at 10 mo. This was associated with recovery of previously abolished somatosensory-evoked potentials. At the end of the study, histological images suggested spinal cord regeneration.ConclusionsOur present findings suggest the possible utility of perilesional intrathecal administration of autologous BMSC in patients with chronic paraplegia. |
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