Transplantation of porcine embryonic stem cells and their derived neuronal progenitors in a spinal cord injury rat model |
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| Authors: | Jenn-Rong Yang Chia-Hsin Liao Cheng-Yoong Pang Lynn Ling-Huei Huang Yi-Ling Chen Yow-Ling Shiue Lih-Ren Chen |
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| Institution: | 1. Division of Physiology, Livestock Research Institute, Council of Agriculture, Executive Yuan, Tainan, Taiwan;2. Department of Medical Research, Buddhist Tzu-Chi University, Hualien, Taiwan;3. Institute of Medical Science, Buddhist Tzu-Chi University, Hualien, Taiwan;4. Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan;5. Institute of Clinical Medicine, National Cheng Kung University, Tainan, Taiwan;6. Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung, Taiwan;7. Institute of Biotechnology, Southern Taiwan University, Tainan, Taiwan;1. Department of Clinical Sciences Malmö, Anaesthesiology and Intensive Care Medicine, Lund University, Skåne University Hospital, Malmö, Sweden;2. Department of Veterans Affairs Medical Center, Center for Imaging of Neurodegenerative Diseases, San Francisco, CA, USA;3. Department of Clinical Sciences Malmö, Clinical Memory Research Unit, Lund University, Skåne University Hospital, SE-20502 Malmö, Sweden;4. UCL Institute of Neurology, London, UK;5. Center for Clinical Research Sörmland, Uppsala University, Eskilstuna, Sweden;6. Department of Clinical Sciences Malmö, Vascular Center, Skåne University Hospital, Malmö, Sweden;1. Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Kanagawa, Japan;2. Department of Orthopaedic Surgery, National Center for Child Health and Development, Setagaya-ku, Tokyo, Japan |
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| Abstract: | Background aimsThe purpose of this study was to investigate therapeutic potential of green fluorescent protein expressing porcine embryonic stem (pES/GFP+) cells in A rat model of spinal cord injury (SCI).MethodsUndifferentiated pES/GFP+ cells and their neuronal differentiation derivatives were transplanted into the contused spinal cord of the Long Evans rat, and in situ development of the cells was determined by using a live animal fluorescence optical imaging system every 15 days. After pES/GFP+ cell transplantation, the behavior functional recovery of the SCI rats was assessed with the Basso, Beattie, and Bresnahan Locomotor Rating Scale (BBB scale), and the growth and differentiation of the grafted pES/GFP+ cells in the SCI rats were analyzed by immunohistochemical staining.ResultsThe relative green fluorescent protein expression level was decreased for 3 months after transplantation. The pES/GFP+-derived cells positively stained with neural specific antibodies of anti-NFL, anti-MBP, anti-SYP and anti-Tuj 1 were detected at the transplanted position. The SCI rats grafted with the D18 neuronal progenitors showed a significant functional recovery of hindlimbs and exhibited the highest BBB scale score of 15.20 ± 1.43 at week 24. The SCI rats treated with pES/GFP+-derived neural progenitors demonstrated a better functional recovery.ConclusionsTransplantation of porcine embryonic stem (pES)-derived D18 neuronal progenitors has treatment potential for SCI, and functional behavior improvement of grafted pES-derived cells in SCI model rats suggests the potential for further application of pES cells in the study of replacement medicine and functionally degenerative pathologies. |
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