Mesenchymal stromal/stem cells markers in the human bone marrow |
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| Authors: | Valeria Rasini Massimo Dominici Torsten Kluba Georg Siegel Giulia Lusenti Hinnak Northoff Edwin M. Horwitz Richard Schäfer |
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| Affiliation: | 1. Department of Oncology, Hematology and Respiratory Diseases, University Hospital of Modena and Reggio Emilia, Modena, Italy;2. Department of Orthopedics, University Hospital Tübingen, Tübingen, Germany;3. Institute of Clinical and Experimental Transfusion Medicine (IKET), University Hospital Tübingen, Tübingen, Germany;4. Division of Oncology/Blood and Marrow Transplantation, The Children''s Hospital of Philadelphia and The University of Pennsylvania School of Medicine, Philadelphia, PA, USA;5. Department of Neurosurgery, Stanford University School of Medicine, Stanford University, Stanford, CA, USA;1. Department of Periodontology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China;2. Guangdong Provincial Key Laboratory of Stomatology, China;1. Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA;2. Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA;1. Division of Pathology, Department of Surgery, University of Cagliari, Cagliari, Italy;2. NICU Center and Institute of Puericulture, Department of Surgery, University of Cagliari, Cagliari, Italy;4. Grupo de Fisiología Celular y Molecular, Facultad de Medicina, Universidad Nacional de Colombia, Carrera 30, No. 45-03, Bogotá D.C., Colombia;5. Instituto de Investigaciones Biomédicas, Facultad de Medicina, Universidad Nacional de Colombia, Carrera 30, No. 45-03, Bogotá D.C., Colombia;1. Department of Hematology, Tianjin Medical University Cancer Hospital and Institute, Tianjin, China;2. Department of Hematology, First Affiliated Hospital of General Hospital of the Chinese People''s Liberation Army, Beijing, China |
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| Abstract: | Background aimsMesenchymal stromal/stem cells (MSCs) can be isolated from human bone marrow (BM), expanded ex vivo and identified via numerous surface antigens. Despite the importance of these cells in regenerative therapy programs, it is unclear whether the cell membrane signature defining MSC preparations ex vivo is determined during culture or may reflect an in vivo counterpart. BM-MSC phenotype in vivo requires further investigation.MethodsTo characterize cells in their natural BM environment, we performed multi-parametric immunohistochemistry on trabecular bone biopsy specimens from multiple donors and described cells by different morphology and micro-anatomic localization in relationship to a precise pattern of MSC antigen expression.ResultsMicroscopically examined high-power field marrow sections revealed an overlapping in vivo expression of antigens characterizing ex vivo expanded BM-MSCs, including CD10, CD73, CD140b, CD146, GD2 and CD271. Expanding this panel to proteins associated with pluripotency, such as Oct4, Nanog and SSEA-4, we were able to identify different cellular populations in the human trabecular bone and BM expressing different progenitor cell markers.ConclusionsTargeting several multipotency and pluripotency markers, we found that the BM contains identifiable and distinct progenitor cells further justifying their introduction for a wide range of applications in regenerative medicine. |
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