Periodontal tissue regeneration by combined implantation of adipose tissue-derived stem cells and platelet-rich plasma in a canine model |
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| Authors: | Morikuni Tobita Cagri A Uysal Xin Guo Hiko Hyakusoku Hiroshi Mizuno |
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| Institution: | 1. Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Tokyo, Japan;2. Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan;1. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China;2. Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China;3. Stem Cells and Regeneration Program, School of Biomedical Sciences, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China;4. MOE Key Laboratory of Regenerative Medicine, School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China;1. UCL Centre for Nanotechnology and Regenerative Medicine, Division of Surgical and Interventional Sciences, University College London, London, United Kingdom;2. Royal Free London NHS Foundation Trust Hospital, London, United Kingdom;1. Dental Material Research Center, Tehran Dental Branch, Islamic Azad University, Tehran, Iran;2. Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran;3. Endodontology Research Group, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff, UK;1. Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, California, USA;2. Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, California, USA;3. Department of Ophthalmology & Vision Science, School of Medicine, University of California, Davis, California, USA;1. Department of Endodontics, National University of Córdoba, Córdoba, Argentina;2. Private Practice, Cetraro, Italy;3. Department of Endodontics, New York University, New York, New York |
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| Abstract: | Background aimsOne goal of periodontal therapy is to regenerate periodontal tissues. Stem cells, growth factors and scaffolds and biomaterials are vital for the restoration of the architecture and function of complex tissues. Adipose tissue-derived stem cells (ASCs) are an ideal population of stem cells for practical regenerative medicine. In addition, platelet-rich plasma (PRP) can be useful for its ability to stimulate tissue regeneration. PRP contains various growth factors and may be useful as a cell carrier in stem cell therapies. The purpose of this study was to determine whether a mixture of ASCs and PRP promoted periodontal tissue regeneration in a canine model.MethodsAutologous ASCs and PRP were implanted into areas with periodontal tissue defects. Periodontal tissue defects that received PRP alone or non-implantation were also examined. Histologic, immunohistologic and x-ray studies were performed 1 or 2 months after implantation. The amount of newly formed bone and the scale of newly formed cementum in the region of the periodontal tissue defect were analyzed on tissue sections.ResultsThe areas of newly formed bone and cementum were greater 2 months after implantation of ASCs and PRP than at 1 month after implantation, and the radiopacity in the region of the periodontal tissue defect increased markedly by 2 months after implantation. The ASCs and PRP group exhibited periodontal tissue with the correct architecture, including alveolar bone, cementum-like structures and periodontal ligament-like structures, by 2 months after implantation.ConclusionsThese findings suggest that a combination of autologous ASCs and PRP promotes periodontal tissue regeneration that develops the appropriate architecture for this complex tissue. |
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