Spleen cells from animals neonatally tolerant to H-2Kk antigens recognize trinitrophenyl-modified H-2Kk spleen cells |
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Authors: | James Forman Jan Klein J Wayne Streilein |
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Institution: | (1) Department of Microbiology and Department of Cell Biology, The University of Texas,Southwestern Medical School at Dallas, 5323 Harry Hines Blvd., 75235 Dallas, Texas |
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Abstract: | A.TL mice injected with (A.AL × A.TL)F1 cells within 24 hours after birth were rendered tolerant to H-2Kk antigens, as evidenced by acceptance of A.TL skin grafts. When spleen cells from these tolerant animals were cocultured with A.AL stimulator cells, no cytotoxic effector cells were generated in a cell-mediated lympholysis assay. However, when the A.AL stimulator cells were derivatized with trinitrophenol, effector cells that displayed a cytotoxic effect against trinitrophenyl-modified H-2Kk target cells were generated. These data indicate that animals tolerant to H-2 determinants but chimeric to only a minor extent possess cytotoxic precursor cells in sufficient frequency to mount a primary in vitro response against trinitrophenol in the context of an allogeneicH-2K region. |
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