Chemical and biological characterization of lipopolysaccharides from the Pseudomonas syringae pv. maculicola IMV 381 collection culture and its dissociants |
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Authors: | G. M. Zdorovenko L. D. Varbanets E. L. Zdorovenko N. V. Vinarskaya L. M. Yakovleva |
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Affiliation: | (1) Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, 03143 Kiev, Ukraine;(2) Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii pr. 47, 119991 Moscow, Russia |
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Abstract: | Lipopolysaccharides (LPS) were isolated from the crude bacterial mass of the Pseudomonas syringae pv. maculicola IMV 381 collection culture and its virulent and avirulent subcultures isolated earlier from the heterogeneous collection culture due to its natural variability during long-term storage. The composition, immunochemical properties, and certain parameters of the biological activity of the LPS preparations obtained were studied. The structural parts of the LPS macromolecule—lipid A, the core oligosaccharide, and O-specific polysaccharide (OPS)—were isolated and characterized. The following fatty acids were identified in the lipid A composition of all cultures: 3-OH-C10:0, C12:0, 2-OH-C12:0, 3-OH-C12:0, C16:1, C16:0, C18:1, and C18:0. Glucosamine (GlcN), ethanolamine (EtN), phosphoethanolamine (EtN-P), and phosphorus (P) were revealed in the hydrophilic portion of the macromolecule. In the core portion of the LPS macromolecule, glucose (Glc), rhamnose (Rha), GlcN, galactosamine (GalN), 2-keto-3-deoxyoctulosonic acid (KDO), alanine (Ala), and P were found. The peculiarities of the structure of LPS isolated from the stable collection culture (LPSstab) and its virulent (LPSvir) and avirulent (LPSavir) subcultures were studied. LPSvir and LPSavir were identical in the monosaccharide composition and contained as the main components L-rhamnose (L-Rha) and 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc), like LPSstab, studied earlier. The NMR spectra of LPSvir were identical to the spectra of LPSstab, whose O-chain repeating unit structure was studied by us earlier, whereas LPSavir differed from LPSvir in the NMR spectrum and was identified by us as the SR form. LPSavir was serologically identical to LPSstab and LPSvir. Hence, the degree of polymerism of the LPS O-chain of P. Syringae pv. maculicola IMV 381 is the main virulence factor in infected model plants. Serological relationships were studied between P. Syringae pv. maculicola IMV 381 and the strains of other pathovars with structurally similar LPS.Translated from Mikrobiologiya, Vol. 73, No. 6, 2004, pp. 790–801.Original Russian Text Copyright © 2004 by G. Zdorovenko, Varbanets, E. Zdorovenko, Vinarskaya, Yakovleva. |
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Keywords: | Pseudomonas syringae dissociation virulence lipopolysaccharide O-specific polysaccharide core oligosaccharide lipid A composition structure biological activity |
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