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Reconstitution of plastoquinone in the D1/D2/cytochrome b-559 photosystem II reaction centre complex
Authors:Kleoniki Gounaris   David J. Chapman  James Barber
Abstract:Reconstitution of plastoquinone in the photosystem II D1/D2/cytochrome b-559 reaction centre complex, in the presence of the detergent Triton X-100, is reported. Illumination of the reconstituted system results in the reduction of cytochrome b-559, the process being partly herbicide-sensitive. In addition, the reconstitution of plastoquinone results in the ability of the isolated reaction centre to catalyse the photoreduction of 2,6-dichlorophenolindophenol in the presence of the exogenous electron donor diphenylcarbazide.
Keywords:Photosynthesis   Photosystem II   Reaction center   Plastoquinone   Reconstitution   Cytochrome b-559
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