Determination of 5‐hydroxytryptamine in serum by electrochemiluminescence detection with the aid of capillary electrophoresis |
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| Authors: | Yuefang Hu Wei Xu Jianping Li Lanjuan Li |
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| Institution: | 1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, , Hangzhou, 310003 People's Republic of China;2. College of Chemistry and Bioengineering, Guilin University of Technology, , Guilin, 541004 People's Republic of China |
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| Abstract: | A rapid, sensitive and simple electrochemiluminescence method for the determination of 5‐hydroxytryptamine (5‐HT) using capillary electrophoresis was proposed. The experimental parameters, including the detection potential, the concentration of Ru(bpy)32+, the concentration and pH of phosphate buffer for separation and detection, the injection voltage and time and the separation voltage on the determination of 5‐HT, were optimized. Under the optimized conditions, the linear concentration range for 5‐HT was 3.5 × 10‐9–5.1 × 10‐3 mol/L, with a detection limit of 5 × 10‐10 mol/L. The relative standard deviations (RSDs) of the ECL intensity and the migration times for six continuous injections of 1.0 µmol/L 5‐HT were 2.48% and 1.3%, respectively. The method was successfully applied to 5‐HT assay in samples of human serum in 5 min and the extraction recoveries with spiked serum samples were over 94.4%. Copyright © 2011 John Wiley & Sons, Ltd. |
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| Keywords: | 5‐hydroxytryptamine electrochemiluminescence capillary electrophoresis Tris(2 2′ ‐bipyridyl) ruthenium(II) |
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