The metabolism of l[3-3H]lactate by isolated hamster liver cells |
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Authors: | Robert Rognstad Pat Wals |
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Affiliation: | Cedars-Sinai Medical Research Institute, Los Angeles, Calif. 90029 U.S.A. |
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Abstract: | The rate of tritium removal from l3-3H]lactate by hamster liver cells is faster than the analytical rate of lactate utilization, or the rate of 14C disappearance from lU-14C]lactate, with the result that the 3H/14C ratio in residual lactate from l-U-14C,3-3H]lactate decreases. However, addition of low concentrations (0.1 to 1.0 mM) of l-cycloserine, a glutamate pyruvate transaminase inhibitor, nearly equalizes the rates of isotope utilization from l-3-3H]lactate and l-U-14C]lactate. The results suggest a very limited rate of recycling of phosphoenolpyruvate back to pyruvate during gluconeogenesis from lactate in fasted hamster liver cells. |
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