ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis |
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Authors: | Rozenn Le Hir Clément Sorin Dipankar Chakraborti Thomas Moritz Hubert Schaller Frédérique Tellier Stéphanie Robert Halima Morin Laszlo Bako Catherine Bellini |
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Affiliation: | 1. Ume? Plant Science Centre, Department of Plant Physiology, Ume? University, S–90183 Ume?, Sweden;2. Ume? Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S–90187 Ume?, Sweden;3. Départements Réseaux Métaboliques Végétaux, Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Université de Strasbourg, 28 rue Goethe, 67083 Strasbourg Cedex, France;4. UMR 1318, AgroParisTech, Institut Jean‐Pierre Bourgin, Institut National de la Recherche Agronomique Centre de Versailles, RD10, 78026 Versailles Cedex, France;5. Plateforme de Cytologie et Imagerie Végétale, UMR 1318, Institut National de la Recherche Agronomique Centre de Versailles, 78026 Versailles Cedex, France |
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Abstract: | In order to obtain insights into the regulatory pathways controlling phloem development, we characterized three genes encoding membrane proteins from the G sub‐family of ABC transporters (ABCG9, ABCG11 and ABCG14), whose expression in the phloem has been confirmed. Mutations in the genes encoding these dimerizing ‘half transporters’ are semi‐dominant and result in vascular patterning defects in cotyledons and the floral stem. Co‐immunoprecipitation and bimolecular fluorescence complementation experiments demonstrated that these proteins dimerize, either by flexible pairing (ABCG11 and ABCG9) or by forming strict heterodimers (ABCG14). In addition, metabolome analyses and measurement of sterol ester contents in the mutants suggested that ABCG9, ABCG11 and ABCG14 are involved in lipid/sterol homeostasis regulation. Our results show that these three ABCG genes are required for proper vascular development in Arabidopsis thaliana. |
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Keywords: | vascular development ABC transporters ABCG dimer lipid/sterol homeostasis
Arabidopsis thaliana
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