Demonstration of adenylate cyclase activity in human red blood cell ghosts |
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Authors: | SB Rodan GA Rodan RI Shaafi |
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Institution: | Departments of Oral Biology and Physiology, University of Connecticut Health Center, Farmington, Conn. 06032, U.S.A. |
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Abstract: | The presence of adenylate cyclase (ATP pyrophosphate-lyase (cyclizing) EC 4.6.1.1) activity was demonstrated in human erythrocyte ghosts and was found to be around 3 pmol adenosine ′,5′-monophosphatase (cyclic AMP) · 2 h?1 · mg?1 protein. This enzymatic activity is strongly stimulated by NaF and 5′-guanylimidodiphosphate, is slightly stimulated by epinephrine, norephrine, soproterenol, and prostaglandin E, and is inhibited by calcium. The hormone stimulation is not potentiated by 5′-guanylylimidodiphosphate. |
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Keywords: | Gpp(NH)p 5′-guanylylimidodiphosphate |
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