Triamcinolone up‐regulates GLUT 1 and GLUT 3 expression in cultured human placental endothelial cells |
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Authors: | Dijle Kipmen‐Korgun Asli Ozmen Gozde Unek Mehmet Simsek Ramazan Demir Emin Turkay Korgun |
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Institution: | 1. Department of Biochemistry, Medical Faculty, Akdeniz University, , Antalya, Turkey;2. Department of Histology and Embryology, Medical Faculty, Akdeniz University, , Antalya, Turkey;3. Department of Obstetrics and Gynecology, Medical Faculty, Akdeniz University, , Antalya, Turkey |
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Abstract: | The placenta is a glucocorticoid target organ, and glucocorticoids (GCs) are essential for the development and maturation of fetal organs. They are widely used for treatment of a variety of diseases during pregnancy. In various tissues, GCs have regulated by glucose transport systems; however, their effects on glucose transporters in the human placental endothelial cells (HPECs) are unknown. In the present study, HPECs were cultured 24 h in the presence or absence of 0·5, 5 and 50 µmol·l–1 of synthetic GC triamcinolone (TA). The glucose carrier proteins GLUT 1, GLUT 3 and GC receptor (GR) were detected in the HPECs. We showed increased expression of GLUT 1 and GLUT 3 proteins and messenger RNA (mRNA) levels (p < 0·05) after 24‐h cell culture in the presence of 0·5, 5 and 50 µmol·l‐1 of TA. In contrast, GR protein and mRNA expressions were down‐regulated (p < 0·05) with 0·5, 5 and 50 µmol·l–1 of TA 24‐h cell culture. The results demonstrate that GCs are potent regulators of placental GLUT 1 and GLUT 3 expression through GR. Excessive exposure to GCs causes maternal and fetal hypoglycemia and diminished fetal growth. We speculate that to compensate for fetal hypoglycemia and diminished fetal growth, the expression of placental endothelial glucose transporters might be increased. Copyright © 2011 John Wiley & Sons, Ltd. |
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Keywords: | glucocorticoids glucose transport pregnancy fetal growth |
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