戊二醛复合消毒剂对猪病病毒杀灭效果的研究

目的　研究戊二醛复合消毒剂对猪病病毒的杀灭效果。方法　将猪细小病毒 (porcinepavrovirusvirus ,PPV)、猪繁殖呼吸综合征病毒 (porcinereproductiveandrespiratorysyndromevirus,PRRSV)和猪伪狂犬病病毒(porcinepseudorabiesvirus,PRV)分别与不同浓度的戊二醛复合消毒剂作用后 ,接种于长满细胞单层的细胞培养板中 ,根据细胞是否产生病变情况 ,确定消毒剂杀灭病毒的最佳浓度 ;同时 ,还将不同浓度的消毒剂与猪瘟病毒 (hogchloeravirus,HCV)作用后 ,对兔进行静脉注射 ,根据兔的体温变化情况 ,判断消毒剂杀灭病毒的最佳浓度。结果　1%浓度的消毒剂 ,可在 2 0min之内有效杀灭PPV、PRRSV和PRV ;0 5 %浓度的消毒剂 ,可在 10min之内有效杀灭HCV。结论　戊二醛复合消毒剂具有高效杀灭病毒的作用 ,适用于养殖场、医疗卫生行业及传染病流行地区的消毒灭菌 ;采用细胞感染法检测和评价消毒剂对病毒的杀灭效果是一种可行的体外试验方法

The Sterilization Effect of Glutaraldehyde Compound Disinfectant on Some Swine Viruses

Objective To study the sterilization effect of glutaraldehyde compound disinfectant on some swine viruses, and to determine the optimum disinfectant concentration.Methods\ PPV,PRRSV and PRV,treated with glutaraldehyde compound disinfectant at different concentration,were inoculated on cell monolayers.The optimum disinfectant concentration was determined according to the cytopathologic effects.HCV reacted with the disinfectant at different concentration was inoculated into rabbits through vein injection,the optimum concentration was decided according to the body temperature changes.Results\ PPV, PRRSV and PRV could be disinfected effectively by 1% disinfectant in 20 min.And HCV could be killed effectively by 0\^5% disinfectant in 10 min.Conclusion\ Glutaraldehyde compound disinfectant possesses good sterilization effect with the concentration of 1% and 0\^5% to some swine viruses,and is suitable for disinfecting the stock farm,hospital and epidemic area of infectious diseases.It is a kind of practicable method of in vitro experiment to detect and judge the sterilization effect of disinfectant with the cell infection method.