Fluorescence resonance energy transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria |
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Authors: | Thijs Kaper Ida Lager Loren L Looger Diane Chermak Wolf B Frommer |
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Institution: | Department of Plant Biology, Carnegie Institution of Washington, Panama Street, Stanford, CA 94305, USA. thijs.kaper@danisco.com |
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Abstract: | Background Engineering microorganisms to improve metabolite flux requires detailed knowledge of the concentrations and flux rates of
metabolites and metabolic intermediates in vivo. Fluorescence resonance energy transfer sensors represent a promising technology for measuring metabolite levels and corresponding
rate changes in live cells. These sensors have been applied successfully in mammalian and plant cells but potentially could
also be used to monitor steady-state levels of metabolites in microorganisms using fluorimetric assays. Sensors for hexose
and pentose carbohydrates could help in the development of fermentative microorganisms, for example, for biofuels applications.
Arabinose is one of the carbohydrates to be monitored during biofuels production from lignocellulose, while maltose is an
important degradation product of starch that is relevant for starch-derived biofuels production. |
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