Chitin-coated Celite as an affinity adsorbent for high-performance liquid chromatography of lysozyme |
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| Authors: | H Yamada T Fukumura Y Ito T Imoto |
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| Affiliation: | 1. Department of Physical and Chemical Sciences, Elizade University, Ilara-Mokin, Nigeria;2. Biocatalysis and Technical Biology Research Group, Institute of Biomedical and Microbial Biotechnology, Cape Peninsula University of Technology, South Africa;3. Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria;4. Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic, Troy, NY 12180, USA;1. Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;2. Electron Microscope Unit, Zoology Department, Science College, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;3. Electron Microscope and Thin Films Department, Physics division, National Research Center, El-Behooth Street, Dokki, Giza 12622, Egypt;4. Pre-Treatment and Finishing of Cellulosic Fabric Department, Textile Research Division, National Research Center, 33 El- Behooth St, Dokki, Giza 12311, Egypt;5. Advanced Materials Research Chair, Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;6. Department of Chemistry, Faculty of Science, Alexandria University, P.O. Box 426, Ibrahimia, Alexandria 21321, Egypt;1. Departamento de Ciências Patológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil;2. EMBRAPA Suínos e Aves, Concórdia, SC, Brazil;3. Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil |
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| Abstract: | Preparation of chitin-coated Celite as an affinity adsorbent for high-performance liquid chromatography of lysozymes and its application to separation of N-bromosuccinimide-oxidized lysozymes are described. By pH gradient elution, two diastereomers of oxindolealanine-62-lysozyme, delta 1-acetoxytryptophan-62-lysozyme (intermediate product in the reaction in acetate buffer), and native lysozyme were all separated within 40 min. |
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