Detection and quantitative determination of abscisic acid by immunological assay |
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| Authors: | Fuchs Yoram Mayak Shimon Fuchs Sara |
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| Institution: | (1) The Volcani Institute of Agricultural Research, Bet Dagan, Israel;(2) Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel |
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| Abstract: | Summary Antibodies with specificity towards abscisic acid (ABA) were produced in rabbits. These antibodies were used for assaying ABA by the inhibition of inactivation of modified bacteriophage. For this assay conjugates of ABA with bacteriophage T4 were prepared and characterized. Such chemically modified bacteriophages were completely inactivated by the specific anti-ABA serum and this inactivation was inhibited by free ABA. The identification and quantitative determination of ABA in plant extracts by this method are demonstrated and the method is compared with a common bioassay. |
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