Rapid attainment of a doubled haploid line from transgenic maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.) plants by means of anther culture |
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Authors: | I?E?Aulinger S?O?Peter J?E?Schmid Email author" target="_blank">P?StampEmail author |
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Institution: | (1) Institute of Plant Sciences, Swiss Federal Institute of Technology ETH, Universitaetstrasse 2, 8092 Zurich, Switzerland |
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Abstract: | Summary We present a strategy for establishing a transgenic doubled haploid maize line from heterozygous transgenic material by means
of anther culture. Compared to conventional inbreeding, the in vitro androgenesis technique enables a faster generation of virtually fully homozygous lines. Since the androgenic response is
highly genotype-dependent, we crossed transgenic, non-androgenic plants carrying a herbicide resistance marker gene (pat, encoding for phosphinothricin acetyl transferase) with a highly androgenic genotype. The transgenic progenies were used
as donor plants for anther culture. One transgenic and three non-transgenic doubled haploid lines have been established within
approximately 1 yr. The homozygosity of all four doubled haploid lines was tested by analysis of simple sequence repeat (SSR)
markers at 19 different loci. Polymorphisms were found between the lines but not within the lines indicating the homozygous
nature of the entire plant genome gained by anther culture. Southern blot analysis revealed that the transgenic donor plants
and their doubled haploid progeny exhibited the same integration pattern of the pat gene. No segregation of the herbicide resistance trait has been observed among the progeny of the transgenic doubled haploid
line. |
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Keywords: | androgenesis homozygosity SSR pat gene herbicide resistance |
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