Characterization of a novel plant acyl-CoA synthetase that is expressed in lipogenic tissues of Brassica napus L. |
| |
Authors: | Pongdontri Paweena Hills Matthew |
| |
Institution: | (1) John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK;(2) Present address: Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand |
| |
Abstract: | A cDNA encoding a novel isoform of acyl-CoA synthetase (ACS6) was isolated from embryos of oilseed rape. Homology searches show it is most closely related to ACS4 from rat and human brain rather than the other oilseed rape ACSs. The ACS6 is strongly expressed in embryos and flowers, tissues of Brassica napus that synthesize lipids at high rates. The activity of recombinantly expressed ACS6 was recovered in the insoluble fraction (214 000×g, 1 h pellet). CHAPS-solubilized recombinant ACS6 protein preferred utilising long-chain fatty acids that contained a cis-9 double bond, i.e. palmitoleic, oleic, linoleic and linolenic acids. Western blot analysis showed that the ACS6 protein is membrane-bound. |
| |
Keywords: | developing seed embryo fatty acid lipid synthesis long-chain acyl-CoA synthetase membrane oilseed rape |
本文献已被 SpringerLink 等数据库收录! |
|