Phenanthrene Biodegradation in Soils Using an Antarctic Bacterial Consortium |
| |
Authors: | Lucas A M Ruberto Susana C Vazquez Antonio Curtosi Marí a C Mestre Emilien Pelletier Walter P Mac Cormack |
| |
Institution: | 1. Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica , Universidad de Buenos Aires , Junín Buenos Aires, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Buenos Aires, Argentina;3. Instituto Antártico Argentino , Buenos Aires, Argentina;4. Institut des Sciences de la Mer de Rimouski (ISMER), Université du Québec à Rimouski , Rimouski, Canada |
| |
Abstract: | Biodegradation of polycyclic aromatic hydrocarbons (PAHs) in Antarctic soils is limited by low temperatures, lack of adequate levels of nutrients, low number of PAH-tolerant members in the autochthonous microbiota and low bioavailability of contaminants. In the present work, microcosms systems (performed in 1-L glass flasks containing Antarctic soil supplemented with 1744 ppm of phenanthrene) were used to study (i) the effect of biostimulation with a complex organic source of nutrients (fish meal) combined with a surfactant (Brij 700); (ii) the effect of bioaugmentation with a psychrotolerant PAH-degrading bacterial consortium (M10); (iii) the effect of the combination of both strategies. The authors found that combination of biostimulation and bioaugmentation caused a significant removal (46.6%) of phenanthrene after 56 days under Antarctic environmental conditions. When bioaugmentation or biostimulation were applied separately, nonsignificant reduction in phenanthrene concentration was observed. Microtox test showed a low increase in toxicity only in the most efficient system. Results proved that “in situ” bioremediation process of phenanthrene-contaminated soils is possible in Antarctic stations. In addition, inoculation with a psychrotolerant PAH-degrading bacterial consortium in association with a mix of fish meal and a high-molecular-weight surfactant improved phenanthrene removal and should be the selected strategy when the number of hydrocarbons degrading bacteria in the target soil is low. |
| |
Keywords: | antarctic bacteria bioaugmentation biostimulation hydrocarbon degradation phenanthrene psychrotolerant bacterial consortium |
本文献已被 InformaWorld 等数据库收录! |
|