Pluripotent stem cell-derived neural stem cells: From basic research to applications |
| |
| Authors: | Masahiro Otsu Takashi Nakayama Nobuo Inoue |
| |
| Institution: | Masahiro Otsu, Department of Chemistry, Kyorin University School of Medicine, Shinkawa, Mitaka , Tokyo 181-8611, Japan;Takashi Nakayama, Department of Biochemistry, Yokohama City University School of Medicine, Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;Nobuo Inoue, Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Higashiogu, Arakawa-ku, Tokyo 116-8551, Japan |
| |
| Abstract: | Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological development of genomic reprogramming to generate induced-pluripotent stem cells, and improvements in vitro techniques to manipulate stem cells. This review summarizes the techniques required to generate neural cells from pluripotent stem cells. In particular, this review describes current research applications of a simple neural differentiation method, the neural stem sphere method, which we developed. |
| |
| Keywords: | Pluripotent stem cells Embryonic stem cells Neural stem cells Neural Stem Sphere method Cell-based therapies |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《World journal of stem cells》浏览原始摘要信息 |
|
