Digital Microfluidic Processing of Mammalian Embryos for Vitrification |
| |
| Authors: | Derek G. Pyne Jun Liu Mohamed Abdelgawad Yu Sun |
| |
| Affiliation: | 1. Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada.; 2. Department of Mechanical Engineering, Assiut University, Assiut, Egypt.; University of Illinois at Chicago, United States of America, |
| |
| Abstract: | Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos. |
| |
| Keywords: | |
|
|
