Isolation and properties of the soluble c-type cytochromes of the dinoflagellate Peridinium cinctum |
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| Authors: | A Hochman T Berman B Plotkin A Schejter |
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| Affiliation: | 1. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia;2. ARC-MQ Industrial Transformation Training Centre (ITTC) for Molecular Technology in the Food Industry, Sydney, Australia;3. Biomolecular Discovery and Design Research Centre, Macquarie University, Sydney, Australia;4. Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia;5. Australian Proteome Analysis Facility, Macquarie University, Sydney, Australia;6. School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Australia;7. Agritechnology Pty Ltd, Borenore, Australia |
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| Abstract: | Four soluble cytochromes of the c type were isolated from the freshwater dinoflagellate Peridinium cinctum collected from Lake Kinneret, Israel. Cytochrome c with alpha-band maximum at 550 nm in the reduced state had a molecular mass of 10,200 Da, pI 7.4, and Em of 278 m V. This cytochrome was active in the respiratory chain of beef heart Keilin-Hartree particles. Cytochrome c-553 had a molecular mass of 13,200 Da, pI 4.9, and Em of 384 m V, and was active in light induced electron transport of Euglena gracilis chloroplast fragments. Cytochrome c-554 had a molecular mass of 13,500 Da, pI 4.4, and Em of 326 m V. This cytochrome was inactive in light induced electron transport but competed with cytochrome c-552 of Euglena in the assay. The acidic cytochrome c-557 was present in very small quantities. The properties of the soluble c-type cytochromes of P. cinctum are compatible with the classification of dinoflagellates as primitive eucaryotes. |
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