Application of the Plackett-Burman experimental design to evaluate nutritional requirements for the production of Colletotrichum coccodes spores |
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Authors: | X Yu S G Hallett J Sheppard A K Watson |
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Institution: | (1) Department of Plant Science, Macdonald Campus of McGill University, Ste-Anne-de-Bellevue, Québec, H9X 3V9, Canada. Fax: (514) 398 7897 e-mail: WATSONA@AGRADM.LAN.MCGILL.CA, CA;(2) Department of Agriculture and Biosystems Engineering, Macdonald Campus of McGill university, Ste-Anne-de-Bellevue, Québec H9x 3V9, Canada, CA |
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Abstract: | Colletotrichum coccodes is being examined as a biological weed control agent for velvetleaf (Abutilon theophrasti). A modified Richard's solution containing V-8 juice has been used to produce C. coccodes spores for growth-chamber and field experiments. Although C. coccodes sporulates well in this medium, V-8 is not available as a bulk commodity and is too expensive for commercial production.
Eight substrates were evaluated as replacements for V-8 juice in modified Richard's solution. Soy protein and casamino acids
were equal to V-8 juice for sporulation of C. coccodes. The Plackett-Burman experimental design was used to test the relative importance of various components of a complex medium
based on soy protein on mycelium biomass production and sporulation of C. coccodes. A new medium composed of sucrose (20 g/l), soy protein (5 g/l), KNO3 (5 g/l), KH2PO4 (5 g/l), MgSO4 (2 g/l), CaCl2 (0.5 g/l), and CuSO4 (0.05 g/l) was selected as the base medium for further study in the development of a low-cost and effective medium for C. coccodes spore production.
Received: 29 July 1996 / Received revision: 21 October 1996 / Accepted: 10 November 1996 |
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