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Evaluation of radical scavenging potential and total phenol content in Woodfordia fruticosa from different altitudes
Authors:Palak Akshyesh Chaturvedi  Arindam Alok Ghatak  Neetin Shivajirao Desai
Institution:1. Department of Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil University, Sector No-15, Plot no 50, CBD-Belapur, Navi Mumbai, 400 614, India
Abstract:Woodfordia fruticosa Kurz is a member of family Lythraceae, commonly used in the treatment of diseases like leucorrhoea, dysentery, leprosy and menorrhagia. The plant material such as leaves, bark and flowers were collected from three different (200, 402 and 600?m) altitudes of Khandala (M.S. India) and the extracts were prepared in solvents like methanol, ethanol and distilled water. The radical scavenging potential and total phenolic content of the extracts were evaluated. The methanol extract of bark collected from the location one (200?m) showed high radical scavenging activity (96.52?±?0.02) than distilled water and ethanol extracts (57.80?±?0.2 and 86.52?±?0.03). The bark of the plant showed highest (663?±?37.85) total phenolic content that of flowers and leaves. The methanol extracts of leaves, bark and flowers showed high tannic acid content, while TBARS assay of ethanol extract of flowers showed maximum protection (27.65). It was observed that there is no significant difference in percent scavenging activity in different plant parts collected from three different altitudes but showed difference in the solvent system used. The results obtained are in support of extensive use of Woodfordia fruticosa in traditional medicine and endorses the use of bark, while it needs further investigations on the plants growing in different geographic areas.
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